You are working in a lab that investigates MAP kinase signaling. The lab has identified a novel s… Show more You are working in a lab that investigates MAP kinase signaling. The lab has identified a novel scaffolding protein SCAF-1 that has already been shown to interact with a receptor that activates the MAP kinase pathway. a) before you start investigating SCAF-1, you recall the general MAP kinase activation pathway. What proteins are involved and in what order do they occur between a ligand approaching the cell and MAP kinase activation? b) You suspect SCAF-1 holds MAPK and two other proteins in close proximity for efficient signaling. How would you show experimentally that SCAF-1 and MAPK interact? Include in your answer your expected results. c) Propose one positive control for your experiment (i.e. proteins that you know for certain do interact) and one negative control for your experiment. • Show less