Dan was… Show more Need Help in microbiology asap please explain in detail. Please answer question 2, 6, and 7. Dan was feeling terrible! He knew he should have worn more sunscreen on that trip to Haiti. Sitting on the plane waiting to be allowed off was torture! He was burning up and felt exhausted. And sitting still so long on the plane had made his ankles and shoulders start to ache too. If only they would let him off the plane! He was beginning to feel nauseous and hoped he didn’t vomit all over his fellow passengers. After they finally let him off the plane he rushed to the bathroom to put some cool water on his face and inspect this horrible sunburn. But it didn’t look normal in the mirror…. His skin was covered in red blotchy spots, especially his chest and all the way up his neck. Maybe he was allergic to something? Can allergies cause this heat (he assumed now it was a fever)? He had to go to the doctor, now. LA General was a short cab ride away. As the PA on duty you ask Dan a few questions before making some theories about what’s causing his symptoms. You discover he just got back from Haiti where he visited friends for 2 weeks. His doctor had given him enough doxycycline for the whole trip and he had taken it religiously. His vaccination history included immunizations to typhoid, hepatitis B and tetanus. He also took Bumex. His major complaints were joint pain, nausea, and the growing rash on his trunk. His fever was 101.8. But as you were talking with him you noticed his speech began to slur a bit. This was deeply concerning. 1. What are the top five suspects for Dan’s infectious disease? Justify your answers. 2. You send off for some laboratory tests right away and find the information below. Interpret each of these findings. Does this change your diagnosis? Why? Leukocytes 3400 Lymphocytes 9 Neutrophils 76 Platelets 140,000/mm3 TNFα 562 pg/ml IL-1β 379 pg/ml 3. Dan’s speech became even more slurred and he could no longer move his arms, flaccid paralysis. You ordered an emergency CT scan that revealed encephalitis. Finally the molecular results came back. Has your tentative diagnosis been confirmed? Interpret the test and describe how it works. Real time reverse transcriptase PCR (rRT-PCR) The probe Cy5-ATGCAAACGGCGACCATGCCGTCA—BBQ at E1 position 10433-10456 was detected after 10 cycles The probe JOE-AAGGACTAGMGGTTAGWGGAGACCC—BBQ at 3’UTR position 10610-10634 was not detected after 300 cycles The probe FAM-CTYAGACCAGCTGAAR-BBQ at NS5 position 9304-9320 was not detected after 300 cycles. 5. How did Dan’s innate immune system try to protect him from the infection? (make sure it is specific for this type of attack) 6. Please describe the acquired immune reaction that occurred in response to this infection. (make sure it is specific for this type of attack) 7. What treatment would you prescribe after confirming your original diagnosis? How does each part of that treatment work? How does each part of that treatment work? Are there any known drug resistances to check? • Show less