A) Real time PCR reactions determi… Show more Which of the following statements are not true regarding qRT-PCR? A) Real time PCR reactions determine the point in time during cycling when amplification of a target is first detected B) Real time PCR is used as an end point assay to determine the amount of product generated in a fixed number of cycles C) The higher the starting copy number of the nucleic acid target, the sooner a significant increase in fluorescence is observed D) There are two types of chemistries used to detect PCR products, fluorogenic 5’ nuclease chemistry, and dsDNA binding dyes E) All of these statements are true • Show less