3’P – TGCGA… Show more A DNA template plus primer with the structure 3’P – TGCGAATTAGCGACAT – P – 5′ 5′-P -ATCGGTACGACGCTTAAC-OH-3′ is placed in an in vitro DNA synthesis system, containing a mutant form of E.coli DNA polymerase I that lacks 5′ to 3’exonuclease activity, but has 5′ to 3’polymerase and 3′ to 5′ exonuclease activities. a. (1pts) What should be present in the in vitro synthesis system for reaction to occur? b. ( 1 pts) What will be the structure of the final product? c. (1 pts)What will be the first step in the reaction sequence? • Show less



