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l of phage stock w… Show more You have a phage stock that

Custom Essays biology l of phage stock w… Show more You have a phage stock that

biology

l of phage stock w… Show more You have a phage stock that

l of phage stock w… Show more You have a phage stock that contains 1×10 10 phage per ml. a. Suppose you mix 100 µl of phage stock with Escherichia coli cells in top agar, pour onto a petri dish containing agar, then incubate overnight. How many plaques do you expect to see the next day? b. Suppose you perform a serial dilution as follows: 100 µl of phage stock added to 9.9 ml dilution broth 100 µl of above to 9.9 ml dilution broth 100 µl of above to 9.9 ml dilution broth 1 ml of above to 9 ml dilution broth You mix 100 µl of the final dilution to E. coli cells in top agar, pour onto plate and incubate overnight. How many plaques do you expect to see the next day? Can anyone provide some guidance or assistance in answering this two questions. I’m a bit lost. The process to solve seems straightforward. However, getting there or knowing where to start has me lost. Thank you. • Show less

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