The two gels illustrated below contain dideoxynucleotide DNA-sequencing (see Chapter 7, pages … Show more The two gels illustrated below contain dideoxynucleotide DNA-sequencing (see Chapter 7, pages 250%u2212254) information for a segment of wild-type and mutant DNA corresponding to the N-terminal end of the protein. The start codon and the next five codons are sequenced. DNA strand sequence of wild-type allele read directly from the gel: 5′-T-G-G-C-G-T-A-A-A-G-T-C-T-G-G-C-A-T-C-C-3′ DNA strand sequence of mutant allele read directly from the gel: 5′-T-G-G-C-G-T-A-A-G-T-C-T-G-G-C-A-T-C-C-3′ coding strands of DNA for both wild-type and mutant allele: 3′-ACCGCATTTCAGACCGTAGG-5′ 3′-ACCGCATTCAGACCGTAGG-5′ Question: A. Write out the mRNA sequence encoded by wild-type template DNA strand. Express your answer as a sequence of nucleotides separated by dashes from 5′ to 3′ B. Write out the mRNA sequence encoded by mutant template DNA strand. Express your answer as a sequence of nucleotides separated by dashes from 5′ to 3′ C. Determine the amino acid sequences translated from wild-type mRNA. D. Determine the amino acid sequences translated from mutant mRNA. • Show less



