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The two gels illustrated below contain dideoxynucleotide DNA

Custom Essays biology The two gels illustrated below contain dideoxynucleotide DNA

biology

The two gels illustrated below contain dideoxynucleotide DNA

The two gels illustrated below contain dideoxynucleotide DNA-sequencing (see Chapter 7, pages … Show more The two gels illustrated below contain dideoxynucleotide DNA-sequencing (see Chapter 7, pages 250%u2212254) information for a segment of wild-type and mutant DNA corresponding to the N-terminal end of the protein. The start codon and the next five codons are sequenced. DNA strand sequence of wild-type allele read directly from the gel: 5′-T-G-G-C-G-T-A-A-A-G-T-C-T-G-G-C-A-T-C-C-3′ DNA strand sequence of mutant allele read directly from the gel: 5′-T-G-G-C-G-T-A-A-G-T-C-T-G-G-C-A-T-C-C-3′ coding strands of DNA for both wild-type and mutant allele: 3′-ACCGCATTTCAGACCGTAGG-5′ 3′-ACCGCATTCAGACCGTAGG-5′ Question: A. Write out the mRNA sequence encoded by wild-type template DNA strand. Express your answer as a sequence of nucleotides separated by dashes from 5′ to 3′ B. Write out the mRNA sequence encoded by mutant template DNA strand. Express your answer as a sequence of nucleotides separated by dashes from 5′ to 3′ C. Determine the amino acid sequences translated from wild-type mRNA. D. Determine the amino acid sequences translated from mutant mRNA. • Show less

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